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superscript tm iv vilo tm master mix with ezdnase tm enzyme  (Thermo Fisher)


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    Thermo Fisher superscript tm iv vilo tm master mix with ezdnase tm enzyme
    Superscript Tm Iv Vilo Tm Master Mix With Ezdnase Tm Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+tm+iv+vilo+tm+master+mix/superscript+iv+vilo+master+mix+with+ezdnase+enzyme/pm40628264-433-161-164
    Average 90 stars, based on 1 article reviews
    superscript tm iv vilo tm master mix with ezdnase tm enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
    Article Snippet: Total RNA was extracted from FACS-purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively. .. RNA samples underwent reverse transcription using SuperScript TM IV VILO TM Master Mix (ThermoFisher) with random hexamers to produce cDNA according to manufacturer’s instructions. qPCR analysis was performed using SYBR Green Master Mix (ThermoFisher) for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Supplementary Table ) and measured with a LightCycler TM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels. ..

    Article Title: Genome stability assessment of PRRS vaccine strain with new ARTIC-style sequencing protocol
    Article Snippet: Vaccine batches were diluted at 1:8 in PBS and then the viral RNA was isolated by the NucleoSpin RNA Virus Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instruction. .. Randomly primed reverse transcription was performed using the SuperScript TM IV VILO TM Master Mix (Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer’s instruction. ..

    Article Title: Genome sequencing identifies a large non-coding region deletion of SNX10 causing autosomal recessive osteopetrosis
    Article Snippet: .. Reverse transcription PCR (RT-PCR) was performed with RNA extracted from white blood cells using RiboPureTM RNA Purification Kit (Invitrogen), and cDNA was prepared using SuperScript TM IV VILO TM Master Mix (Invitrogen). ..

    Article Title: A cohesin traffic pattern genetically linked to gene regulation
    Article Snippet: .. Reverse transcription was performed with VILO IV (SuperScript TM IV VILO TM Master Mix, 11756050, Thermo Fisher Scientific) and incubated 20 minutes at 25°C, 10 minutes at 50°C and 5 minutes at 85°C. cDNA was diluted by 20 and 2μL was used for the qPCR. .. qPCR was directly done on the cDNAs using Fast SYBR TM Green Master Mix (ThermoFisher Scientific, 4385612) and analyzed on a StepOnePlus TM Real-Time PCR System (ThermoFisher Scientific) using StepOne TM Plus v2.3 software.

    Article Title: Effects of aged garlic extract on experimental periodontitis in mice
    Article Snippet: Total RNA was extracted using a RNeasy Plus Mini kit (Qiagen GmbH) and quantified using a microvolume spectrophotometer at 260 and 280 nm (NanoDrop-2000; Thermo Fisher Scientific, Inc.). .. The RNA was reverse transcribed to cRNA by using SuperScript TM IV VILO TM Master Mix (Thermo Fisher Scientific, Inc.) at 25 ̊C for 10 min, 42 ̊C for 60 min, and 85 ̊C for 5 min. qPCR with the cDNA was performed using Power SYBR TM Green PCR Master Mix (Thermo Fisher Scientific, Inc.) and specific primers ( ) using 7300 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
    Article Snippet: Total RNA was extracted from FACS-purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively. .. RNA samples underwent reverse transcription using SuperScript TM IV VILO TM Master Mix (ThermoFisher) with random hexamers to produce cDNA according to manufacturer’s instructions. qPCR analysis was performed using SYBR Green Master Mix (ThermoFisher) for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Supplementary Table ) and measured with a LightCycler TM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels. ..

    Article Title: A cohesin traffic pattern genetically linked to gene regulation
    Article Snippet: .. Reverse transcription was performed with VILO IV (SuperScript TM IV VILO TM Master Mix, 11756050, Thermo Fisher Scientific) and incubated 20 minutes at 25°C, 10 minutes at 50°C and 5 minutes at 85°C. cDNA was diluted by 20 and 2μL was used for the qPCR. .. qPCR was directly done on the cDNAs using Fast SYBR TM Green Master Mix (ThermoFisher Scientific, 4385612) and analyzed on a StepOnePlus TM Real-Time PCR System (ThermoFisher Scientific) using StepOne TM Plus v2.3 software.

    Article Title: Effects of aged garlic extract on experimental periodontitis in mice
    Article Snippet: Total RNA was extracted using a RNeasy Plus Mini kit (Qiagen GmbH) and quantified using a microvolume spectrophotometer at 260 and 280 nm (NanoDrop-2000; Thermo Fisher Scientific, Inc.). .. The RNA was reverse transcribed to cRNA by using SuperScript TM IV VILO TM Master Mix (Thermo Fisher Scientific, Inc.) at 25 ̊C for 10 min, 42 ̊C for 60 min, and 85 ̊C for 5 min. qPCR with the cDNA was performed using Power SYBR TM Green PCR Master Mix (Thermo Fisher Scientific, Inc.) and specific primers ( ) using 7300 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. ..

    SYBR Green Assay:

    Article Title: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
    Article Snippet: Total RNA was extracted from FACS-purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively. .. RNA samples underwent reverse transcription using SuperScript TM IV VILO TM Master Mix (ThermoFisher) with random hexamers to produce cDNA according to manufacturer’s instructions. qPCR analysis was performed using SYBR Green Master Mix (ThermoFisher) for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Supplementary Table ) and measured with a LightCycler TM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels. ..

    Variant Assay:

    Article Title: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
    Article Snippet: Total RNA was extracted from FACS-purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively. .. RNA samples underwent reverse transcription using SuperScript TM IV VILO TM Master Mix (ThermoFisher) with random hexamers to produce cDNA according to manufacturer’s instructions. qPCR analysis was performed using SYBR Green Master Mix (ThermoFisher) for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Supplementary Table ) and measured with a LightCycler TM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels. ..

    Expressing:

    Article Title: Dual-targeting CRISPR-CasRx reduces C9orf72 ALS/FTD sense and antisense repeat RNAs in vitro and in vivo
    Article Snippet: Total RNA was extracted from FACS-purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively. .. RNA samples underwent reverse transcription using SuperScript TM IV VILO TM Master Mix (ThermoFisher) with random hexamers to produce cDNA according to manufacturer’s instructions. qPCR analysis was performed using SYBR Green Master Mix (ThermoFisher) for all qPCRs except C9orf72 variant 2 expression levels for which TaqMan probes were used (sequences provided in Supplementary Table ) and measured with a LightCycler TM (Roche). qPCR was performed in technical triplicate with data normalised to GAPDH expression levels. ..

    cDNA Synthesis:

    Article Title: Body Fluid Identification in Samples Collected after Intimate and Social Contact: A Comparison of Two mRNA Profiling Methods and the Additional Information Gained by cSNP Genotypes
    Article Snippet: .. Complementary DNA (cDNA) synthesis of the RNA extracts was performed using SuperScript TM IV VILO TM Master Mix (invitrogen by Thermo Fisher Scientific, Waltham, MA, USA), following the manufacturer’s protocol. ..

    Article Title: Two Avastrovirus Species Discovered in Psittaciformes Expand the Host Range of the Family Astroviridae
    Article Snippet: .. Briefly, samples underwent ezDNase treatment (Invitrogen), FastSelect HMR & Bacteria & globin mRNA removal (QIAGEN), single-strand cDNA synthesis with SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, USA), and second-strand cDNA synthesis with Sequenase (Thermo Fisher Scientific, Waltham, MA, USA). ds-cDNA was cleaned up with Mag-Bind ® Total Pure NGS (Omega Bio-tek, Norcross, GA, USA). .. Library preparation included tagmentation and indexation using Nextera XT DNA Library Prep Kit with unique dual indexes (Illumina, San Diego, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Genome sequencing identifies a large non-coding region deletion of SNX10 causing autosomal recessive osteopetrosis
    Article Snippet: .. Reverse transcription PCR (RT-PCR) was performed with RNA extracted from white blood cells using RiboPureTM RNA Purification Kit (Invitrogen), and cDNA was prepared using SuperScript TM IV VILO TM Master Mix (Invitrogen). ..

    Article Title: Effects of aged garlic extract on experimental periodontitis in mice
    Article Snippet: Total RNA was extracted using a RNeasy Plus Mini kit (Qiagen GmbH) and quantified using a microvolume spectrophotometer at 260 and 280 nm (NanoDrop-2000; Thermo Fisher Scientific, Inc.). .. The RNA was reverse transcribed to cRNA by using SuperScript TM IV VILO TM Master Mix (Thermo Fisher Scientific, Inc.) at 25 ̊C for 10 min, 42 ̊C for 60 min, and 85 ̊C for 5 min. qPCR with the cDNA was performed using Power SYBR TM Green PCR Master Mix (Thermo Fisher Scientific, Inc.) and specific primers ( ) using 7300 Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. ..

    Purification:

    Article Title: Genome sequencing identifies a large non-coding region deletion of SNX10 causing autosomal recessive osteopetrosis
    Article Snippet: .. Reverse transcription PCR (RT-PCR) was performed with RNA extracted from white blood cells using RiboPureTM RNA Purification Kit (Invitrogen), and cDNA was prepared using SuperScript TM IV VILO TM Master Mix (Invitrogen). ..

    Incubation:

    Article Title: A cohesin traffic pattern genetically linked to gene regulation
    Article Snippet: .. Reverse transcription was performed with VILO IV (SuperScript TM IV VILO TM Master Mix, 11756050, Thermo Fisher Scientific) and incubated 20 minutes at 25°C, 10 minutes at 50°C and 5 minutes at 85°C. cDNA was diluted by 20 and 2μL was used for the qPCR. .. qPCR was directly done on the cDNAs using Fast SYBR TM Green Master Mix (ThermoFisher Scientific, 4385612) and analyzed on a StepOnePlus TM Real-Time PCR System (ThermoFisher Scientific) using StepOne TM Plus v2.3 software.

    other:

    Article Title: MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome
    Article Snippet: RNA was quantified using DS-11 (DeNovix, Wilmington, DE).

    Bacteria:

    Article Title: Two Avastrovirus Species Discovered in Psittaciformes Expand the Host Range of the Family Astroviridae
    Article Snippet: .. Briefly, samples underwent ezDNase treatment (Invitrogen), FastSelect HMR & Bacteria & globin mRNA removal (QIAGEN), single-strand cDNA synthesis with SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, USA), and second-strand cDNA synthesis with Sequenase (Thermo Fisher Scientific, Waltham, MA, USA). ds-cDNA was cleaned up with Mag-Bind ® Total Pure NGS (Omega Bio-tek, Norcross, GA, USA). .. Library preparation included tagmentation and indexation using Nextera XT DNA Library Prep Kit with unique dual indexes (Illumina, San Diego, CA, USA).

    Next-Generation Sequencing:

    Article Title: Two Avastrovirus Species Discovered in Psittaciformes Expand the Host Range of the Family Astroviridae
    Article Snippet: .. Briefly, samples underwent ezDNase treatment (Invitrogen), FastSelect HMR & Bacteria & globin mRNA removal (QIAGEN), single-strand cDNA synthesis with SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, USA), and second-strand cDNA synthesis with Sequenase (Thermo Fisher Scientific, Waltham, MA, USA). ds-cDNA was cleaned up with Mag-Bind ® Total Pure NGS (Omega Bio-tek, Norcross, GA, USA). .. Library preparation included tagmentation and indexation using Nextera XT DNA Library Prep Kit with unique dual indexes (Illumina, San Diego, CA, USA).



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